MCS – MOM Computer System and Software
The MOM Computer System (MCS) includes the software package MScan. This program has been designed to seamlessly control two-photon imaging using conventional or resonant scanners while incorporating photostimulation and electrophysiology.
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The MOM Computer System (MCS) includes the software package MScan. This program has been designed to seamlessly control two-photon imaging using conventional or resonant scanners while incorporating photostimulation and electrophysiology. While designed exclusively for use with the MOM microscope, it is also compatible with other two-photon platforms. The MCS is designed to take on complex experiments in deep-tissue intravital imaging. Its intuitive user interface is easy to use. The MCS package and the MOM together form a formidable tool to understand the most complex issues in neuroscience, immunology or oncology. Importantly, you will find in MCS the same standard of technical excellence that is the hallmark of all Sutter Instrument products.
The MScan 2.0 software has been developed to simplify the many tasks inherent in complicated imaging experiments. MScan 2.0 is extensively multithreaded to take advantage of multicore processors. This ensures reliability and user interface responsiveness. Furthermore, MScan is multiuser based to facilitate sharing of a MOM microscope with MCS among experimenters. Experimenters can then send their data to other workstations for analysis. The MCS analysis program MView is available for free download on the Sutter Instrument website.
MCS includes a windows 7 workstation, National Instruments data acquisition boards, a USB camera and a USB controlled MPC-200. The National Instruments boards included are a PCI-6110 board for imaging, a PCIe-6353 board for control of imaging and photostimulation laser power and a PCIe-6321 board for electrophysiology. The package is a turnkey system as all data acquisition boards and software come installed within the workstation.
An important feature in MScan is the ability to do bidirectional frame scanning with sub-pixel line offset adjustment. Conventional two-photon frame scanning has involved unidirectional scanning. In these scenarios, data is only recorded when sweeping in one direction across the sample. To increase the rate of data acquisition, it is then necessary to steer the laser beam back to the origin of the scan as quickly as possible to begin the subsequent line. As galvanometric scanners are most taxed and most likely to be damaged during these high-frequency movements, bidirectional scanning both increases the speed at which frames can be recorded and decreases the likelihood of damage to expensive galvanometers.
Features
- Large-scale, high-resolution, deep-tissue mapping
- Multispectral, high-speed, functional optical imaging
- Resonant scanning capable with imaging rates to 30 frames per second
- Photostimulation while imaging through the same optical pathway (“photostimaging”)
- Two-photon microscopy and concurrent electrophysiology with computer-controlled placement of electrodes by Sutter micromanipulators
Common Applications
- Two-photon imaging
- Deep tissue imaging
- Neurophysiology
- Immunology
- Embryology
Ordering Information
MCS System
| Catalog Number | Description |
| MOM-MCS* | System includes preinstalled MCS2.0 software, Windows 7 workstation, data acquisition boards, USB camera |
* Must be used with MOM systems equipped with MOM-DAQ, MPC-200 and ROE-200
MCS Components
| Catalog Number | Description |
| MOM-DAQ | MOM data acquisition system (includes NI 6110E PCI board) |
| MPC-200-ROE | Includes MPC-200 controller and ROE-200 |
NOTE: The MOM-MCS communicates with the MPC-200 controller via the USB port for control of X, Y, and Z movement. It is not compatible with the MP-285 controller. Please contact Sutter for more information.
Technical Information
| Imaging Modes
XY movie – Real-time continuous zoom and rotation, 1° increment XZ movie – Requires Z-piezo nanopositioner Timelapse Stack – Synchronized Z focus and power modulation (linear or exponential) Fast stack – Fast XYZT time series when used with Z-piezo nanopositioners Line scan – User-drawn trajectory with arbitrary orientation and position Region scan – User-designated collections of points, lines, rectangles, ellipses or polygons Photostimulation scan – User-designated collections of points, lines, rectangles, ellipses or polygons 3-D Map Window Stores multispectral frames or stacks in a 25 x 25 x 25 mm 3-D world in objective coordinates Functional Imaging Real-time display of averaged intensities of regions of interest (ROIs) in scrolling plot Analog Inputs 8 analog channels, up to 250 kHz continuous acquisition rate Software Stimulator Ideal to interface electrical or optogenetic stimulators Targeted Patch-Clamping Support for one Sutter Instrument micromanipulator Cellular Amplifier Control Automatic real-time scaling of inputs via telegraph gain |
Imaging Features
4 imaging channels with independent gains and user-adjustable pixel duration Bidirectional line scan with sub-pixel line offset adjustment User or TTL triggered Control of Imaging Power Dedicated fast analog output (sub-microsecond response time) to control laser intensity via Pockels cell allowing beam blanking on scan turnabout Power Measurement Interfaces with an optical laser power meter for accurate, real-time measurement of laser power to the preparation Focus Control Full XYZ control of objective placement with Sutter Instrument MPC-200 Photostimulation Dedicated fast analog output (sub-microsecond response time) to control laser intensity via: Photostimaging Photostimaging is photostimulation during imaging. Photostimaging can be enabled during an XY movie, line scan or region scan Integrated Development Environment Rich object model to control hardware, fully compatible with ActiveX Automation MCS Data Files Designed with GLP (Good Laboratory Practive) compliant header, visible from Windows shell Data File Management Semi-automatic backup of data files at the end of a session at up to two different locations (i.e. thumb drive, network disk…) Bloggable Notebook Automatic log of events accessible via the built-in web browser |
Dimensions (subject to change)
CPU
20.5 in x 8.25 in x 20 in | 52 cm x 21 cm x 51 cm
Monitor
26 in x 10 in x 19 in | 67 cm x 24 cm x 48 cm
Weight (subject to change)
CPU
31 lbs | 14 kg
Monitor
15 lbs | 7 kg
Electrical
115/ 230 Volts
50/ 60 Hertz power line
RoHS compliant
