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Krumdieck Tissue Slicer
Brain, Organ Slicing
The instrument provides samples of uniform and reproducible thickness, minimizes damage to surfaces of slices and thereby eliminates the main sources of error in tissue slice work. Slices from approximately 100 to 500 microns in thickness can be prepared at a maximum rate of one slice every 3 to 4 seconds. The slicer operates submerged in a buffer selected by the operator as most appropriate in terms of composition, tonicity, pH, temperature, oxygenation, and lubricating properties to maintain the viability of the tissues being sectioned. The microtome and the reservoir with the glass trap are sterilizable to allow the preparation of aseptic slices suitable for prolonged organ culture. The actual slicing is done by a rapidly reciprocating disposable blade oscillating at 1000 to 2500 rpm driven by a motor that also powers the impeller. The tissue slicer contains a cooling block which allows the temperature of the buffer solution in the slicer’s reservoir to be maintained.
Features
- Separate arm and blade motor speed control
- Reduced vibrations through shorter counterbalanced drive shaft
- Elimination of blade chatter
- Micro-adjustment of blade’s edge sample platform gap
- Only commercially available sterilizable microtome
Selected Publications
Kim, K.H., Kim, J., Han, J.Y. et al. In vitro estimation of metal-induced disturbance in chicken gut-oviduct chemokine circuit. Mol. Cell. Toxicol. 15, 443–452 (2019).
Ramzan, A.A., Bitler, B.G., Hicks, D. et al. Adiponectin receptor agonist AdipoRon induces apoptotic cell death and suppresses proliferation in human ovarian cancer cells. Mol Cell Biochem 461, 37–46 (2019).
Fu, Y., Tong, J., Meng, F. et al. Ciliostasis of airway epithelial cells facilitates influenza A virus infection. Vet Res 49, 65 (2018).
Selected Product Citations
An integrated multiomic and quantitative label-free microscopy-based approach to study pro-fibrotic signalling in ex vivo human precision-cut lung slices |
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| Muzamil Majid Khan, Daniel Poeckel, …, Rainer Pepperkok | Eur Respir J | Published 1 Jul 2021 |
| Article Snippet Briefly, on the day the tissue resection sample was received (day 0), lung tissue was inflated with 3% low melting agarose (Sigma #A9414) prepared in phenol-free DMEM (Gibco #41965-039).. Next, 8 mm cores were prepared and 250-μm-thick slices were generated using a Krumdieck tissue slicer (TSE Systems, Bad Homburg, Germany).. To facilitate recovery following the slicing procedure, the tissue medium was supplemented with penicillin, streptomycin, fungizone and 10% FCS for the first 18 h of ex vivo culture. |
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| Figure Legend as tumour-free by the local pathologist. 4: Tumour-free tissue was received and inflated with 3% low melting agarose. 5: Tissue cores of varying heights were prepared. 6: Human precision-cut lung slices (hPCLS) of 8 mm diameter and 250 µm thickness were prepared using a Krumdieck tissue slicer. On day 0, each hPCLS generated was distributed across a 24-well plate (1 hPCLS·well −1 ). For all the days of culture, each hPCLS was cultured in 750 µL of DMEM with constant presence of antimicrobials (amphotericin B and penicillin–streptomycin). From day 0 to day 1 (18 h), the tissue was kept in DMEM with 10% FCS. 7: On day 1, old media (with FCS) was replaced with fresh media (no FCS). Media (with no FCS) was also replenished on days 4, 7 and 10. When required, hPCLS were additionally treated with transforming growth factor-β1 (TGF-β1) or any other stimulant from day 1 … |
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Streptococcus suis Induces Expression of Cyclooxygenase-2 in Porcine Lung Tissue |
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| Muriel Dresen, Josephine Schenk, …, Andreas Nerlich | Microorganisms | Published 12 Feb 2021 |
| Article Snippet After solidifying on ice, cylindrical portions of lung tissue were punched out with a tissue coring tool so that the bronchus/bronchiole was in the middle.. The approximately 300-µm thick slices were cut by a Krumdieck tissue slicer (model MD 4000-01; TSE Systems, Chesterfield, MO, USA).. PCLS were collected in RPMI-1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) containing antibiotics and antimycotics (1 µg/mL clotrimazole, 10 µg/mL enrofloxacin (Bayer, Leverkusen, Germany), 50 µg/mL kanamycin, 100 U/mL penicillin, 100 µg/mL streptomycin/mL, 50 µg/mL gentamicin, 2.5 µg/mL amphotericin B). |
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Bordetella bronchiseptica promotes adherence, colonization, and cytotoxicity of Streptococcus suis in a porcine precision-cut lung slice model |
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| Désirée Vötsch, Maren Willenborg, …, Peter Valentin-Weigand | Virulence | Published 29 Dec 2020 |
| Article Snippet As previously described [ , , ], the cranial, middle, and intermediate lung lobes were filled with 1.5% (w/v) low-melting agarose (GERBU, Heidelberg, Germany) in RPMI 1640 medium (Sigma-Aldrich, Taufkirchen, Germany) and kept on ice until agarose became solidified.. Afterward, cylindrical pieces of lung tissue with a bronchiole in the middle were punched out with a tissue-coring tool and cut into approximately 300 µm thick slices using a Krumdieck tissue slicer (model MD 4000–01; TSE Systems, Chesterfield, MO, USA).. Slices were collected in RPMI 1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with antibiotics and antimycotics (1 µg/ml clotrimazole, 10 µg/ml enrofloxacin, 50 µg/ml kanamycin,100 U/ml penicillin, 100 µg/ml streptomycin, 50 µg/ml gentamicin, 2.5 µg/ml amphotericin B) and bubbled for approximately 2 hours (h) with a.. |
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