R811 Dual Colour Multichannel Fibre Photometry System
R811 dual color multichannel fiber photometry system is compatible with optoelectronics equipment. It uses two wavelengths of excited light, 410nm and 470nm, of which 410 is used to acquire reference signal and eliminate noise. The system can record signals of green fluorescence indicators like GCaMP and dLight or neurotransmitter probes.
The R811 sysem can record neural signals from 9 sites at the same time.
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Overview
The dual colour fibre photometry system records changes in the fluorescence intensity of neurons in a specific brain area to reflect neuronal population activity. In the study of neural circuits, the fiber photometry system can perform long-term stable monitoring of the neurons of freely moving animals, and explore the correlation between neural activity and animal behavior.
Main Applications
Ca2+ and neurotransmitter signal detection
Study the function of neural circuits
Explore the mechanisms of neurological diseases
Development of new fluorescent sensor probes
Experiment of optical principles
Advantages
Two frequencies of light, 410 nm and 470 nm, are respectively used for excitation of reference and green fluorescence ;
Support up to 9 channels, suitable for simultaneous experiment of multiple animals or multiple brain locations;
Professional acquisition and analysis softwares are flexible and easy to operate with data processing functions available. No matlab programming is required;
Supports multiple acquisition modes including continuous acquisition, interval acquisition, acquisition upon trigger, delayed acquisition and timing acquisition;
Live display of DeltaF/F acquisition to check scale of signal changes during acquisition;
Customized adjustment of output signal parameter, easily trigger and control external excitation equipment to achieve closed-cycle control of excitation and recording;
Compatible with optogenetics for recording and stimulation at the same site.
Professional Acquisition and Analysis Software
Professional acquisition and analysis software enable stable data acquisition and easy processing. Data analysis
includes data clipping, bleaching correction, smoothing, movement correction, event heat map, peak statistics,
area under curve and heat map of behavior trajectory.
Generation of Peri-event heat map with one click. Freely choose and handle events of interest, and flexibly add or remove events. The results can be easily saved and exported to DetaF/F, Z-score, Peri-event, peak statistics and AUC. Information in the image can be freely edited by saving the image as editable SVG format.
Accessories
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Optical Fibre |
Fibre Optic Cannulae with Ceramic Ferrule
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Ceramic Sleeves
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Ceramic Ferrules Protective Cap
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Laser Power Meter
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Ceramic Ferrule Holder
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| Wavelength of excitation light | 410nm 470nm |
| Power | Min 0 µW, Max ≥ 100 µW, adjustable with an accuracy of 0.1 µW |
| Number of channels | 9 |
| Frame rate of fluorescent sampling | Max 250fps |
| Digital signal interface | 4 Input and 4 Output |
| Signal output | Output frequency 0-500Hz, adjustable output pulse width and duration |
| Marking | Manual marking (10), automatic marking (4), ROI marking (9) |
| Behavior camera | 1920*1080(30fps) 1280*720(60fps) . Switchable among multiple frame rates of resolution |
| Compatible | Compatible with optogenetics for recording and stimulation at the same site |
