R821 Tricolour Multichannel Fibre Photometry System
R821 tricolour multichannel fibre photometry system is compatible with optoelectronics equipment. It uses three wavelengths of excited light, 410nm and 470nm, of which 410 is used to acquire reference signal and eliminate noise. The system can record signals of green fluorescence indicators such as GCaMP and dLight or neurotransmitter probes. and red fluorescence indicators such as RCaMP, jrGECO1a or neurotransmitter probes.
The R821 sysem can record neural signals from 9 sites at the same time.
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Overview
The R821 tricolour multichannel fibre photometry system records changes in the fluorescence intensity of neurons in a specific brain area to reflect neuronal population activity. In the study of neural circuits, the fibre photometry system can perform long-term stable monitoring of the neurons of freely moving animals, and explore the correlation between neural activity and animal behavior.
Main Applications
Ca2 + and neurotransmitter signal detection
Study the function of neural circuits
Explore the mechanisms of neurological diseases
Develop new fluorescent sensor probe
Experiment of optical principle
Advantages
Three kinds of excitation light sources, that is 410nm、470nm and 560nm, are respectively used for excitation of reference, green fluorescence and red fluorescence;
Support up to 9 channels, suitable for simultaneous experiment of multiple animals or multiple brain locations;
Dual highly sensitive detectors enabling independent and sequential detection to avoid interference of fluorescence excitation and detection, acquiring more accurate signal;
Professional acquisition and analysis softwares are flexible and easy to operate with data processing functions available. No matlab programming is required;
Supports multiple acquisition modes including continuous acquisition, interval acquisition, acquisition upon trigger, delayed acquisition and timing acquisition;
Live display of DeltaF/F acquisition to check scale of signal changes during acquisition;
Customized adjustment of output signal parameter, easily trigger and control external excitation equipment to achieve closed-cycle control of excitation and recording;
Compatible with optogenetics for recording and stimulation at the same site.
References:
Journal：Nature Neuroscience
Demand of the Product：We recorded calcium dynamics in DG-projecting SuM neurons by fiber photometry. In vivo recordings were carried out in an
open-top HC (21.6×17.8×12.7 cm3) or EE (45×25× 20 cm3) containing toys, colorful balls, domes or tunnels and food, for 10min.
Cite Reference：Li, YD., Luo, YJ., Chen, ZK. et al. Hypothalamic modulation of adult hippocampal neurogenesis in mice confers activity-dependent regulation of memory and anxiety-like behavior. Nat Neurosci 25, 630–645 (2022). https://doi.org/10.1038/s41593-022-01065-x
Journal：Cell Stem Cell
Demand of the Product：We performed fiber photometry to record calcium activity of CA3 and CA1 pyramidal neurons labeled with GCaMP6f upon chemogenetic activation of SuM-enhanced ABNs by intraperitoneal CNO injection.
Cite Reference：Li YD, Luo YJ, Xie L, Tart DS, Sheehy RN, Zhang L, Coleman LG Jr, Chen X, Song J. Activation of hypothalamic-enhanced adult-born neurons restores cognitive and affective function in Alzheimer’s disease. Cell Stem Cell. 2023 Apr 6;30(4):415-432.e6. doi: 10.1016/j.stem.2023.02.006
| Wavelength of excitation light | 410nm 470nm 560nm |
| Power | Min 0 µW, Max ≥ 100 µW, adjustable with an accuracy of 0.1 µW |
| Number of channels | 9 |
| Frame rate of fluorescent sampling | Max 250fps |
| Digital signal interface | 4 Input and 4 Output |
| Signal output | Output frequency 0-500Hz, adjustable output pulse width and duration |
| Marking | Manual marking (10), automatic marking (4), ROI marking (9) |
| Behavior camera | 1920*1080(30fps) 1280*720(60fps) . Switchable among multiple frame rates of resolution |
| Compatible | Compatible with optogenetics for recording and stimulation at the same site |
